During metaphase replicated chromosomes (each possessing two sister chromatids attached to each other at centromere via proteins) align on the metaphase plate. Sister chromatids separate during anaphase and become individual chromosomes that migrate towards spindle poles. Chromosomes arrive at spindle poles during telophase where nuclear envelop reforms and chromosomes relax. Finally, cytokinesis takes place and two daughter cells, each carrying an equal number of chromosomes, form.

This process is different from meiosis, where homologous pairs are initially attached and exchange information. Pairs segregate during miosis I. Next, sister chromatids separate during miosis II to produce four daughter cells, each carrying haploid chromosome sets.