barkoulas2016
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- | ===== Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene Expression in Caenorhabditis. ===== | + | ===== cis-regulatory motifs ===== |
+ | **Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene Expression in Caenorhabditis** | ||
Barkoulas et al. PLoS Genet 2016 Sep; 12(9): | Barkoulas et al. PLoS Genet 2016 Sep; 12(9): | ||
PMID: 27588814 DOI: 10.1371/ | PMID: 27588814 DOI: 10.1371/ | ||
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Comparative studies have shown that changes in cis-regulatory sequences contribute to gene expression differences. However, cis evolution may also occur without altering gene expression. | Comparative studies have shown that changes in cis-regulatory sequences contribute to gene expression differences. However, cis evolution may also occur without altering gene expression. | ||
- | In this paper, authors have investigated the regulation of //lin-3// (EGF-family) in Rhabditidae nematode species, and report significant cis changes within a 58-bp cell-specific enhancer fragment even though the enhancer activates lin-3 expression in a conserved manner. //lin-3// was originally identified in Caenorhabditis elegans and shown to encode for a secreted EGF ligand that induces vulva formation. The ligand is produced by a specialized cell in the gonad, termed anchor cell. The findings show that the 58-bp region has undergone cis evolutionary changes in nematodes, resulting in the turnover of major transcription factor binding sites. Heterologous transgene expression studies involving //C. elegans// and two distant species //C. angaria// and //C. afra// uncovered compensatory evolution in //lin-3// enhancer. What makes this work more interesting is the in vivo quantification of cell-specific transcription and the use of CRISPR/Cas9 genome editing technology in a multicellular organism to manipulate endogenous gene expression. | + | In this paper, authors have investigated the regulation of //lin-3// (EGF-family) in Rhabditidae nematode species, and report significant cis changes within a 58-bp cell-specific enhancer fragment even though the enhancer activates lin-3 expression in a conserved manner. |
+ | === === | ||
+ | //lin-3// was originally identified in // | ||
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+ | What makes this work more interesting is the //in vivo// quantification of cell-specific transcription and the use of CRISPR/Cas9 genome editing technology in a multicellular organism to manipulate endogenous gene expression. |
barkoulas2016.1492353359.txt.gz · Last modified: 2017/04/16 14:35 by 5omw78